En metod för detektering och kvantifiering av gamma-hydroxysmörsyra (GHB) i urin med gaskromatografi (GC) är framtagen på Sahlgrenska universitetssjukhuset. Metoden är relativt unik då den inte kräver upparbetning i form av derivatisering, indunstning eller extraktion. Urinen surgörs med koncentrerad saltsyra och internstandard, gamma-valerolakton, tillsätts. GHB övergår då till laktonformen, gamma-butyrolakton (GBL). Därefter injiceras provet direkt på en GC-FID med en kapillärkolonn för glykoler och alkoholer.

In this project a LC-MS-method for the analysis of gamma-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, amphetamine and methadone was developed.Initially, the efficiency of the ionisation of the analytes was evaluated with respect to the ionisation technique (ESI, APCI and APPI) and the composition of the mobile phase. In the next step a number of different columns was tested in order to find the one with the greatest potential for separation of the substances in question. Using the selected column, the separation was optimised by means of experimental design and the software The Unscrambler 7.8. The parameters studied were the flow rate, the column temperature and the mobile phase composition. The response variables were the resolution between the target compounds and the retention time of the last eluting compound.These experiments showed that, in order to obtain the best ionisation, the mobile phase should consist of 5 mM formic acid in water and acetonitrile.

In this dissertation I have tried to figure out if it possible to make any money on acid grain to feed cows through a case study. The method is a case study combined with a documentary research. At present the farm sell all grain in connection with harvest and buy it back continuously in the neighbourhood, I have compared that with an alternative where I invest in a construction for to store acid grain on the current farm. I have delimit me to only have a look on acid grain because I think that is one of the cheapest way too store grain in smaller aplenty. Before beginning with acid grain is it important to be versed in how everything works and the risk to keep on with acid grain. One disadvantage is that the acid grain is not possible to store more than 12 months with out acid it once again. It is also easy to get mould in the pasture if it is not acid enough.

Syftet med studien var att studera effekterna av några miljöfaktorer och genetiskt predisponerande faktorer för reumatoid artrit (RA). RA är en inflammatorisk sjukdom där immunsystemet bryter ner kroppsegen vävnad. En signifikant ökad risk för RA påvisades vid exponering för spannmålsdamm och rökning. Generna som studerades var GSTM1, GSTT1 och Fc-gamma-RII. Generna i sig var ingen predisponerande faktor för RA, men rökning visade sig vara en signifikant högre riskfaktor för de som hade en deletion av GSTM1 eller Fc-gamma-RII genen samt för de som inte hade deletion av GSTT1 genen.

En metod för detektering och kvantifiering av gamma-hydroxysmörsyra (GHB) i urin med gaskromatografi (GC) är framtagen på Sahlgrenska universitetssjukhuset. Metoden är relativt unik då den inte kräver upparbetning i form av derivatisering, indunstning eller extraktion. Urinen surgörs med koncentrerad saltsyra och internstandard, gamma-valerolakton, tillsätts. GHB övergår då till laktonformen, gamma-butyrolakton (GBL). Därefter injiceras provet direkt på en GC-FID med en kapillärkolonn för glykoler och alkoholer.

A sample of milk inoculated with S. aureus was treated with penicillin, and Antipen (a new commercial product containing penicillinase) was added. To another sample with S. aureus and penicillin, formic acid to pH 5 was added, and to yet another sample, a combination of formic acid and Antipen was added. Controls with no penicillin were treated in the same way.

Fermented liquid feed is well known for its health promoting effects on piglets. High levels of lactic acid are desired in the feed together with low levels of acetic acid and certain biogenic amines. Limits for acetic acid have been suggested to be 30-40 mmol/kg to avoid a decreased palatability of the feed; however, few studies have been performed. The purpose of this trial was therefore to examine which levels of lactic acid and acetic acid that can be accepted in a fermented feed without affecting the feed intake and thus the weight gain of the pig. A total of 60 pigs (Yorkshire/ Hampshire) were used in a trial during two weeks, between 9-11 weeks of age.

Increasing attention is being given to acid sulphate soils wherever they occur. The problems that leaching sulphate soils gives with significant lowerings of pH and mobilization of heavy metals influence large spectra of our society from fisheries to agriculture to construction. Mapping these soils is consequently of great importance and the methods of doing this is very much lacking in function and precision. This study was therefore carried out to investigate whether conductivity in running water can be used as a simple instrument to identify acid sulphate soils in the catchment. 31 coastal streams in the county of Västerbotten were analyzed for different catchment properties such as occurence of marine sediments and basic water chemistry including conductivity and sulphate.

One of the most commonly used methods for analysis of polyphenols is the Folin-Ciocalteu (FC) method, where the FC reagent has been reported to interfere with some substances in fruit juices. The interfering substance that is present in the highest amount in most fruit juices is ascorbic acid. In this study, the total phenolic content (TPC) of three fruit juices was analysed by the FC method as well as with an enzymatic method with hydrogen peroxide oxidoreductase type II (HRP) for comparison. To investigate how the interfering ascorbic acid affects the TPC, juice samples were analysed after removal and addition, respectively, of ascorbic acid. The samples were analysed with HPLC both before and after the extraction phase of the FC method.

The first part of the thesis consists of a brief introduction to the general principles of target detection and the sensor-systems used. In the following part there is a theoretical description of the algorithms this thesis focuses on. The detection algorithms described in this paper are called Cell Average, Ordered Statistics, 2parameter and Gammadetector. Two different discriminators called Extended Fractal Features and Quadratic Gamma Discriminator are also described. The algorithms are tested on three different types of data, simulated SAR-pictures, authentic SAR-targets and IR-pictures.

The aim of this study was to investigate if the hen?s genotype, age and diet affect fat components in egg yolk. The fat components measured were total fat content, phospholipids, cholesterol and the fatty acid profile. This trial involved 12 groups of laying hens housed in 8-hen cages enriched with perches, dust baths and nests. The genotype Lohmann Selected Leghorn (LSL) and the genotype Lohmann Brown (LB)was used.

Lymfom är inte vanligt förekommande hos hästar, men det är den mest förekommande maligna tumörformen. Det är en lymfoproliferativ sjukdom som kan uppträda kliniskt på många olika sätt och det finns många olika tumörsubtyper. Det är därför viktigt att kunna definiera lymfom hos häst på ett bra sätt. Det kan göras genom att klassificera sjukdomen i fyra olika kategorier och från dessa kategorier sedan dela in tumörerna i olika subgrupper. De vanligaste subgrupperna hos häst är T-cellsrika B-cellslymfom, perifera T-cellslymfom och diffusa stora B-cellslymfom. Det är inte alltid lätt att diagnostisera lymfom hos häst.

The varieties of paint are today very big, but many of the more popular paints are less than environmental friendly. The uses of fossilized fuel in paints are common because these paints dry fast and are color true. It would be beneficial for the environment if more renewable source were used to produce paint. Linseed oil is an old base for oil paints. It is renewable and easily procured.

Joint injury (inflammation) or osteoarthritis is the most common cause of poor performance in the athletic horse. Osteoarthritis is the ensuing degeneration of joint cartilage due to inflammation/injury. The single most important factor in the inflammation cascade of joint injuries is interleukin-1 (IL-1). Until recently, these injuries have been treated with products like hyaluronic acid, cortisone and polysulphated glycosaminoglycans (PSGAG). Several studies have not been able to demonstrate a significant difference in the clinical improvement of joint injuries treated with hyaluronic acid or PSGAG and saline.

In hydropower plants it is necessary to always have local power supply. Therefore, the plants are equipped with batteries as stationary back up power. Vattenfall Vattenkraft is using lead acid batteries but has been investigating alternatives to replace them. The aim of this study is to investigate the potential of using Li-ion technology as back up power.The study showed that Li-ion batteries have many qualities. The Li-ion technology will decrease the space and maintenance demand.